Chromophobe Kidney Mobile or portable Carcinoma of a Kidney Allograft

25 and 0. 1 ng/mL, and the limits of quantification(LOQ) for 25(OH)D_2, 25(OH)D_3 and vitamin K_1were 3, 0. 75 and 0. 3 ng/mL, respectively. The recoveries of three levels in the matrix were 98. 5%-104. 3%, the relative standard deviation(RSD) were all less than 5. 0%(n=6).
An UPLC-MS/MS method for analysis of 25(OH)D_2, 25(OH)D_3 and vitamin K_1 in serum is sensitive, rapid, accurate and suitable for the nutritional surveillance of vitamin D and K_1 in the population.
An UPLC-MS/MS method for analysis of 25(OH)D_2, 25(OH)D_3 and vitamin K_1 in serum is sensitive, rapid, accurate and suitable for the nutritional surveillance of vitamin D and K_1 in the population.
To establish an analytical method for determination of 6 kinds of α_2-agonists in animal foods by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).
The samples of animal food were enzymatic hydrolysis by β-glucosidase/arylsulfatase, purified by MCX column. The separation was performed on a Dikma leapsil C_(18) column(2. 1 mm×100 mm, 2. 7 μm), then the target compound were detected by ultra high performance liquid chromatography-tandem mass spectrometry with electron spray ionization(ESI) positive ion scan in mode of multiple reaction monitoring(MRM) and quantified by matrix matched external standard method.
At the spiked level of 1, 2 and 4 μg/kg, the recoveries of each compound were in the range of 70. 4%-111. 2% with the relative standard deviations of 2. 3%-18. 8%. The qualitative limits of detections were 0. #link# 06-0. 3 μg/kg and the quantitative limits were 0. 2-1. 0 μg/kg for the 6 targets compounds. By using the established method, the target compound in 30 samples including pork, pig liver, pig kidney, beef and mutton were detected, and no excessive veterinary drug residue were detected.
The established method is simple, rapid, high sensitivity and good stability, with a wide variety and a certain development. It can provide more convenient and fast detection method support for the daily monitoring of veterinary drug residues in animal food.
The established method is simple, rapid, high sensitivity and good stability, with a wide variety and a certain development. It can provide more convenient and fast detection method support for the daily monitoring of veterinary drug residues in animal food.
To establish a method for the simultaneous and rapid determination of vitamin A and vitamin E of different configurations in human serum by high performance liquid chromatography(HPLC) with multi-wavelength fluorescence detector.
The serum was mixed after adding internal standard, and acetonitrile was added for protein precipitation. The mixture was centrifuged after extraction with n-hexane. link2 The n-hexane layer was dried by N_2 flow, the residue was dissolved with methanol. The HPLC system was consisted of WATERS Symmetry C_(18) column(4. 6 mm × 250 mm, 5 μm) and the mobile phase was methanol. The column temperature was 30 ℃ and fluorescence detector with online wavelength conversion method was carried out for the quantitative detection.
The liner range of determination of vitamin A, α-vitamin E, β-vitamin E and δ-vitamin E were 0. 050-2. 0 μg/mL, 0. 50-50 μg/mL, 0. 050-5. 0 μg/mL and 0. 050-5. 0 μg/mL, respectively(r≥0. 996). The minimum detection limits of the method for vitamin A and vitamin E were all 0. 02 μg/mL. The intraday and interday relative standard deviations(RSDs) were less than 3% at high, medium and low concentrations. The recoveries of the samples at the three concentrations were 91. 2%-107. 5%, and the RSDs were less than 10%.
This method is simple and accurate, with higher sensitivity than using UV detector and can be used for the simultaneous determination of vitamin A and vitamin E of different configurations in serum, and is suitable for rapid detection of batch serum samples.
This method is simple and accurate, with higher sensitivity than using UV detector and can be used for the simultaneous determination of vitamin A and vitamin E of different configurations in serum, and is suitable for rapid detection of batch serum samples.
To observe the changes of neuropeptide Y(NPY) expression in perirenal adipose tissue and its relationship with insulin resistance in the nutritional transition models of refeeding after calorie restriction.
SPF Male SD rats, aged 8 weeks, were randomly divided into normal chow group and refeeding with normal chow after calorie restriction for 4 weeks group. NPY gene expression in perirenal adipose tissue were detected by real-time quantitative PCR at the end of 4 and 12 weeks, along with fasting plasma glucose, fasting serum lisulin, free fatty acids and average glucose infusion rate(GIR_(60-120)) of hyperinsulinemic-euglycemic clamp test for 60-120 minutes. NPY gene mRNA expression in perirenal adipose tissue was detected by real-time quantitative PCR. And the relationship between NPY gene expression and insulin resistance was detected by Spearman correlation analysis.
The expression level of NPY gene in perirenal adipose tissue in caloric restriction for 4 weeks group was significantly increased by caThe result of correlation analysis showed that in the 4-week group, the mRNA expression level of NPY gene in perirenal adipose tissue was closely related to GIR_( 60-120)、fasting insulin and free fatty acid, with R values of-0. 765, 0. 716 and 0. 657 respectively(P<0. 01). In the 12 week group, the mRNA expression level of NPY gene in perirenal adipose tissue was closely related to GIR_(60-120), fasting insulin and free fatty acid, with R values of-0. 853, 0. 622 and 0. 608 respectively(P<0. 01).
The mRNA expression of NPY gene in perirenal adipose tissue was closely related to indicators of insulin resistance. It is an important factor affecting insulin sensitivity.
The mRNA expression of NPY gene in perirenal adipose tissue was closely related to indicators of insulin resistance. LY3475070 is an important factor affecting insulin sensitivity.
To evaluate the effects of genetically modified maize with Cry1Ab and epsps genes on immune function in F3 rats.
A total of 180 weaning SD rats for F0 generation were randomly divided into three groups, which were treated with AIN-93 G feed control diet, parental maize diet and genetically modified maize diet respectively. After three generations of breeding, antibody producing cells determination, concanavalin A(ConA)-induced lymphocyte transformation test, natural killer(NK)cells activities assay, whole blood lymphocyte subtype detection, delayed type hypersensitivity test and immunity organ index were performed.
There were no significant differences between parental maize diet and genetically modified maize diet in terms of the number of antibody-producing cells, ConA-induced spleen lymphocyte proliferation, NK cell activity, whole blood lymphocyte subsets, delayed type hypersensitivity and thymus index(P>0. 05).
Under the conditions of this experiment, no significant effects were found on immune function of F3 SD rats through the three generation development study of genetically modified maize with CrylAb and epsps genes.
Under the conditions of this experiment, no significant effects were found on immune function of F3 SD rats through the three generation development study of genetically modified maize with CrylAb and epsps genes.
In order to investigate the effect of yeast on reducing mycotoxin damage in dried fish.
A strain of Meyerozyma guilliermondii MH 211588. 1(MG-81) was mixed and fermented 48 h with dried Lutjanus erythopterus which contaminated aflatoxin B_1(AFB_1) and T-2 toxin(T-2). The toxin concentration in fermentation at different time was detected by LC-MS/MS, and fermentation was fed with mice by intragastric administration(7 d). Blood routine and four liver function enzymes were measured by hematology analyzer and microplate spectrophotometer respectively. The elimination effect of MG-81 isolate on mycotoxin damage in dried fish was evaluated by the toxin concentration at different time and its toxic effect on mice.
The removal rates of AFB_1 and T-2 in dried fish fermentation showed a parabolic linear growth trend with the prolongation of fermentation time. The removal rates of AFB_1 and T-2 in dried fish fermentation broth tended to be stable at 36 h(the removal rates of AFB_1 and T-2 were 83. 7%±1. 3% and 78. 5%±0. 8%). This indicated that 36 h was the optimal time for MG-81 to remove mycotoxins in dried fish. At the same time, it was found that there was no significant change in the indexes of MG-81 dried fish fermentation compared with the control group(P>0. 05), while the same dose of AFB_1 and T-2 dried fish fermentation(without MG-81), the leucocytes, lymphocytes, erythrocyte, hemoglobin, platelet and mean platelet volume of mice were significantly lower than those of control group(P>0. 05), showing obvious hemotoxicity and immunotoxicity. link3 The activity of four liver enzymes was increased significantly(P<0. 05), showing obvious hepatotoxicity.
The fermentation of MG-81 for 36 h can effectively remove AFB_1 and T-2 from dried fish and eliminate their hazards.
The fermentation of MG-81 for 36 h can effectively remove AFB_1 and T-2 from dried fish and eliminate their hazards.
To understand the rural sanitary conditions in different geographical areas of Shaanxi Province.
According to the stratified random sampling method, 30 agriculture-related counties were selected(The central area includes 13 counties in Xi'an, Tongchuan, Weinan, Xianyang and Baoji cities. The southern area includes 10 counties in Hanzhong, Ankang, Shangluo cities. The northern area includes 7 counties in Yulin, Yan'an cities. ). Five townships were selected randomly in each county(excluding Chengguan Town), and 4 administrative villages were selected randomly in each township as survey villages, which were collected the soil samples for testing lead, cadmium and chromium in each subject village, and 5 households were randomly selected in each villages as survey households. The data was obtained through data reading, interviews, on-site observations, and laboratory testing, etc. The detection of soil lead and cadmium was carried out according to the Measurement of Soil Quality Lead and Cade central area than in the southern area and northern area. Sanitation toilets have a low penetration rate in the province, which was higher in the southern area than in the central area and the northern area. The domestic garbage was randomly discarded, and domestic sewage was randomly discharged, which was more in the northern area than in the central area and the southern area. Soil cadmium pollution was relatively heavy, mainly in the southern area.
To explore the role of calcium/calmodulin-dependent protein kinase Ⅱ(CaMK-Ⅱ) and protein phosphatase-2 A(PP2 A) on hyperphosphorylation of tau induced by AlCl_3 in SH-SY5 Y cell.
SH-SY5 Y cells were assigned to the control group, AlCl_3 low, middle, high exposed groups(200, 400800 μmol/L Al~(3+)), KN93 intervention group(800 μmol/L Al~(3+)+0. 5 μmol/L KN93) and sphingosine intervention group(800 μmol/L Al~(3+)+5 nmol/L sphingosine). After 48 h of exposure and intervention, the viabilities of cells were measured by CCK-8 assay, the contents of CaMK-Ⅱ and PP2 A were determined by ELISA, and the expression of tau5 and phosphorylation of Thr-181, Thr-231, Ser-262 and Ser-396 were detected by Western-Blot.
The viabilities of cells in AlCl_3 middle and high exposed groups were significantly lower than that of the control group(P<0. 05). Compared with the AlCl_3 high exposed group, the viabilities of cells in KN93 intervention group and sphingosine intervention group were significantly increased(P<0.