Thermogravimetric Test involving Urea with Continuous Temperature ranges

To explore the impacts of bosentan combined with sildenafil on chronic obstructive pulmonary disease (COPD) patients with pulmonary arterial hypertension (PAH).
From April 2019 to October 2020, 90 COPD patients with PAH diagnosed in our hospital were recruited and divided into groups A and B. The patients in group A (50 cases) were treated with bosentan combined with sildenafil, and the patients in group B (40 cases) were administered bosentan combined with iloprost solution for inhalation. The PAH conditions, the heart rates (HR), the cardiac function, the pulmonary function, the blood gas indexes, the inflammatory factor expressions, the incidences of adverse reactions, the overall response rates (ORR), and the patient satisfaction levels were determined or evaluated.
Compared with group B, the patients in group A had better recovered PAH, HR, cardiac function, pulmonary function, and blood gas indexes, lower inflammatory factor expression levels and a lower incidence of adverse reactions, as well as higher ORR and higher satisfaction levels.
Bosentan combined with sildenafil can reduce pulmonary artery pressure and promote the recovery of cardiopulmonary function in COPD patients with PAH.
Bosentan combined with sildenafil can reduce pulmonary artery pressure and promote the recovery of cardiopulmonary function in COPD patients with PAH.Deep learning (DL)-based convolutional neural networks facilitate more accurate detection and rapid analysis of MLS. Our objective was to assess the feasibility of applying a DL-based convolutional neural network to non-contrast computed tomography (CT) for automated 2D/3D brain midline shift measurement and outcome prediction after spontaneous intracerebral haemorrhage. In this retrospective study, 140 consecutive patients were referred for CT assessment of sICH from January 2014 to April 2019. The level of consciousness of patients was evaluated using the Glasgow Coma Scale (GCS) score, and the Glasgow Outcome Scale (GOS) score was calculated to classify the outcome. The distance of midline shift (MLS-D) and volume of midline shift (MLS-V) were automatically measured via DL methods. Patients were divided into three groups based on GCS scores mild degree (GCS score 13-15), moderate degree (GCS score 9-12), and severe degree (GCS score 3-8). Spearman's correlation analysis revealed statistically significant (P less then 0.01) positive correlation between GCS and MLS-D (r=0.709) and MLS-V (r=0.754). The AUC of MLS-V was slightly larger than that of MLS-D (0.831 vs 0.799, P=0.318) in the midline shifting group. The AUC of MLS-V was significantly larger than that of MLS-D (0.854 vs 0.736, P=0.03) in patients with severe degree GCS scores. The DL-based measurements of both MLS-D and MLS-V enable the assessment of consciousness and the prediction of the outcome of sICH. Compared to MLS-D, MLS-V measurement can better indicate mass effect and predict outcomes, particularly in severe cases.Charcot-Marie-Tooth (CMT) 2A disease, a genetic axonal nervous lesion, results from MFN2 pathogenic variation, and this gene plays a pivotal role in mitochondrial dynamics and calcium signaling. However, the underlying mechanism linking MFN2 defect to progressive dying-back of peripheral nerves is still unclear. The present work focused on analyzing one CMT2A patient from multiple perspectives. Clinical and pathologic evaluation was initially conducted on the recruited case. Subsequently, Sanger sequencing and whole-exome sequencing (WES) were performed for genetic detection. To reveal the cell metabolic alteration caused by the identified variant, this study also established and transfected plasmid vectors in HEK293 cells and analyzed cell metabolites through liquid chromatography in combination with quadrupole time-of-flight tandem mass spectrometry (UPLC Q-TOF MS). Additionally, we completed structural modeling and molecular dynamic (MD) simulation to investigate the intramolecular impact of the variant. According to our results, the clinical and neuropathologic manifestations of the proband matched with the diagnosis of CMT. The causative variant MFN2 c.638T>C (p.Ile213Thr) was identified through genetic analysis. Moreover, metabolic pathway enrichment results demonstrated that this variant significantly affected the metabolism of sphingolipids and glycerophospholipids. MD analysis indicated that this variant crippled the binding ability of MFN2 to GTP. Taken together, our study deduced preliminary clues for the underlying mechanism by which mutant MFN2 affects cell metabolism and provided a novel perspective to understand the cellular and molecular impacts of MFN2 variants.
This study retrospectively analyzed the clinical and imaging features of TM mycosis complicated with bone destruction with the aim to improve understanding, diagnosis, and treatment.
Data of hospitalized TM-infected patients with bone destruction from October 2012 to May 2019 were collected. The clinical and imaging features of the disease were comprehensively analyzed.
All 35 patients were non-HIV infected, but some had underlying co-morbid illnesses. The duration of the disease was 1-36 months (median 5 months). Fever, anemia, weight loss, and respiratory symptoms were the main clinical manifestations of the patients. There were 18 patients (51.4%) who had bone pain. Peripheral blood leukocyte count increased significantly in 27 patients (77.1%). The neutrophil count increased in 28 patients (80%). C-reactive protein (CRP) and immunoglobulin G levels increased in 93.3% (14/15) and 82.1% (23/28) patients, respectively. The imaging examination showed osteolytic lesions, which were multiple in several bony areas.
Young and middle-aged patients with non-AIDS TM complicated with underlying diseases should be especially cautious in case of occurrence of bone destruction. The main clinical manifestations of patients with TM complicated with bone destruction were pulmonary symptoms and bone and joint pain, which could be accompanied by progressive consumptive diseases.
Young and middle-aged patients with non-AIDS TM complicated with underlying diseases should be especially cautious in case of occurrence of bone destruction. THZ1 in vivo The main clinical manifestations of patients with TM complicated with bone destruction were pulmonary symptoms and bone and joint pain, which could be accompanied by progressive consumptive diseases.
To investigate the effects of early restrictive fluid resuscitation (RFR) on the clinical outcomes in sepsis patients.
A total of 122 sepsis patients admitted to our hospital were recruited for this study and divided into a study group (the SG, n=56) and a control group (the CG, n=66) according to the treatment method each patient was administered. The SG was administered early RFR, and the CG was administered adequate fluid resuscitation. The clinical data were analyzed retrospectively in both groups. The total infusion volumes, the hemorrhage amounts, the urine outputs, and the Acute Physiology and Chronic Health Evaluation (APACHE II) scores were compared between the two groups. In addition, the heart rates, the mean arterial pressure levels, the central venous pressure levels, and the cardiac function indices were compared between the two groups at 1-7 days after the procedures. The survival and the complication incidence rates were followed up.
The SG showed significantly lower heart rates and mean patients.
To explore the effect of stapled trans-anal rectal resection (STARR) on constipation symptoms and inflammatory reaction in patients with outlet obstructive constipation (OOC).
From January 2019 to June 2020, a retrospective analysis was conducted on the medical data of 124 patients with OOC admitted to our hospital. According to the different surgical methods, sixty patients were assigned to the control group (CG) treated with Bresler operation, and sixty-four patients were included in the research group (RG) receiving STARR. The total effective rate of treatment, the incidence of postoperative complications, and mid- and long-term recurrence rate were observed between the two groups. The anorectal dynamic indexes, the constipation scoring system (CSS), obstructive defecation syndrome (ODS), visual analogue scale (VAS) scores, and the changes of inflammatory cytokine [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] were compared between the two groups, and the perioperative indexes were observedt to the CG.
STARR can effectively improve the clinical efficacy, ameliorate the symptoms of postoperative constipation, reduce the long-term recurrence rate, relieve postoperative pain, and better protect the anorectal function and mitigate inflammatory reaction for patients with obstructive constipation.
STARR can effectively improve the clinical efficacy, ameliorate the symptoms of postoperative constipation, reduce the long-term recurrence rate, relieve postoperative pain, and better protect the anorectal function and mitigate inflammatory reaction for patients with obstructive constipation.
To investigate the correlations between preoperative diffusion tensor imaging (DTI), a Magnetic Resonance Imaging (MRI)-based technique and surgical outcome in patients with cervical spondylotic myelopathy (CSM).
A retrospective study of 95 patients with CSM who received diagnosis and surgical treatment in our hospital was carried out. According to the recovery rate of the Japanese Orthopaedic Association (JOA) scale at the 1-year postoperative follow-up, the patients were divided into a good recovery group (JOA recovery rate ≥60%, n = 47) and a poor recovery group (JOA recovery rate <60%, n = 48). Patients in both groups underwent diffusion tensor imaging examination before surgery. The preoperative fractional anisotropy (FA) value, apparent diffusion coefficient (ADC) value, longitudinal dispersion (AD) rate, and lateral dispersion (VD) rate were compared between the two groups. Pearson correlation coefficient was used to analyze the correlation between the preoperative DTI quantization parameters (Fvery rate, whereas there was no statistical significance (both P>0.05). The CR, TA, MSCC and MCC values measured before surgery in the good recovery group were significantly lower than those in the poor recovery group (all P<0.001); were negatively correlated with the JOA recovery rate (all P<0.05), while the correlation with TA was not statistically significant (P>0.05).
DTI can evaluate the severity of the patient's condition before surgery by analyzing the subtle structural changes in patients with CSM. At the same time, the preoperative FA, VD, CR, MSCC, and MCC values are all associated with the surgery efficacy, which paves the way for the next step of clinical treatment.
DTI can evaluate the severity of the patient's condition before surgery by analyzing the subtle structural changes in patients with CSM. At the same time, the preoperative FA, VD, CR, MSCC, and MCC values are all associated with the surgery efficacy, which paves the way for the next step of clinical treatment.
To explore the expression levels and the potential regulatory mechanism of miR-21-5p in LPS-treated H9c2 cells.
The secretions of the inflammatory cytokines induced by LPS in H9c2 cells were evaluated using ELISA. We used RT-RCR and western blot to measure the relative mRNA and protein expression levels in LPS-treated H9c2 cells. CCK-8 and EdU assays showed the viability and proliferation profiles of the H9c2 cells. TUNEL assays demonstrated the apoptotic behaviors of the H9c2 cells, and a luciferase reporter analysis was used to investigate the interactions between miR-21-5p and programmed cell death protein 4 (PDCD4).
LPS induced damage to the H9c2 cells by reducing the cell viability and down-regulating miR-21-5p. On the other hand, miR-21-5p overexpression inhibited the LPS-induced inflammatory damage in the H9c2 cells. Moreover, PDCD4 was verified as a downstream target gene of miR-21-5p, and its expression was inhibited by the higher miR-21-5p content. Finally, miR-21-5p inhibited septic processes, and the PDCD4 overexpression rescued the miR-21-5p effect in the LPS-treated H9c2 cells.